International Journal of Immunology

Volume 2, Issue 3, June 2014

  • General Approach for Isolation of Immunoglobulins Fragments with the Core Hinge

    Maria Timchenko, Vladimir Tischenko

    Issue: Volume 2, Issue 3, June 2014
    Pages: 16-23
    Received: Oct. 06, 2014
    Accepted: Nov. 01, 2014
    Published: Nov. 18, 2014
    Abstract: The original limited proteolysis approach was proposed for large (multidomain) proteins with post-translational modifications for obtaining of their novel fragments. It was realized for human immunoglobulins representing two subclasses IgG2 and IgG3. This approach was based on two techniques: masking of protein regions which are normally susceptibl... Show More